Lipid peroxidation and oxidant stress regulate hepatic apolipoprotein B degradation and VLDL production
J. Clin. Invest. Meihui Pan, et al. 113:1277 doi:10.1172/JCI19197 [Go to this article.]

Figure 1
DHA stimulates ApoB100 degradation in primary hepatocytes independent of the LDL receptor. Primary hepatocytes from Ldlr-KO (A) or wild-type (B) C57BL/6J male mice were maintained overnight in modified Waymouth’s medium. Cells were then incubated with either BSA or DHA/BSA complexes and subjected to the pulse-chase protocol described in the text. Chase medium and cell lysate samples were harvested at the indicated time points for immunoprecipitation/SDS-PAGE analysis of newly synthesized ApoB100. The fluorograms shown here on the right, as well as in the following figures, were representative of three independent experiments, in each of which at least duplicate wells were analyzed. The graph summarizes the recovery data of total (cell lysate + medium) labeled ApoB100 as determined by densitometric analysis of the fluorograms. The arrows in this and the other figures indicate the position of the ApoB100 band. *P < 0.001 vs. BSA.