Neurofibromin-deficient Schwann cells secrete a potent migratory stimulus for Nf1+/– mast cells
J. Clin. Invest. Yang Feng-Chun, et al. 112:1851 doi:10.1172/JCI19195 [Go to this article.]

Figure 2
Effect of WT, Nf1+/–, and Nf1–/– Schwann cell CM on WT and Nf1+/– mast cell haptotaxis. (a) Transwells were coated with recombinant FN and mast cell migration assays were performed in response to WT, Nf1+/–, and Nf1–/– Schwann cell CM. The number of WT and Nf1+/– mast cells that had migrated to the bottom surface of the FN-coated membrane in response to Schwann cell CM were counted after staining the cells with crystal violet. A representative photomicrograph of the WT- and Nf1+/–-migrated mast cells, which stain purple, is shown for each experimental condition. (b) The average numbers of WT and Nf1+/– mast cells per ten high-power fields, which migrated in response to either 105 WT, Nf1+/–, or Nf1–/– Schwann cell CM are shown. Data represent the mean number of migrated cells per ten high-power fields ± SEM of four independent experiments. *P < 0.05 for Nf1+/– versus WT mast cells in response to either WT Schwann cell CM or Nf1+/– Schwann cell CM. **P < 0.05 for WT mast cells in response to both WT and Nf1+/– Schwann cell CM versus Nf1–/– Schwann cell CM; ***P < 0.01 for Nf1+/– mast cells in response to both WT and Nf1+/– versus Nf1–/– Schwann cell CM by the Student’s paired t test. HPF, high-power field.