The molecular basis for adhesion-mediated suppression of reactive oxygen species generation by human neutrophils
J. Clin. Invest. Tieming Zhao, et al. 112:1732 doi:10.1172/JCI19108 [
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Figure 7Tyrosine phosphatase inhibition and cytoskeletal disruption restore adhesion-inhibited NADPH oxidase activity and Rac2 and Vav1 activation. Neutrophils treated with vanadate or cytochalasin b (cyto b) as in described in Methods were tested for H
2O
2 production in response to 1 × 10
–7 M fMLF under suspension and adherent conditions (
a). Rac2 (
b) and Vav1 (
c) activities were determined after treatment with fMLF for 5 minutes. Results shown are representative of two independent experiments using cells from different donors.
