The molecular basis for adhesion-mediated suppression of reactive oxygen species generation by human neutrophils
J. Clin. Invest. Tieming Zhao, et al. 112:1732 doi:10.1172/JCI19108 [
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Figure 5Adhesion of neutrophils to fibronectin leads to delayed Vav1 activation but early Syk activation in response to fMLF stimulation. Neutrophils plated on fibronectin (50 μg/ml) or in nonadherent conditions were treated with 1 × 10
–7 M fMLF. After the times indicated, Vav1 and Syk tyrosine phosphorylation (P-Tyr) was determined, as described in Methods. Total Vav1 and Syk levels (anti-Vav1 and anti-Syk IB) were constant with time under each condition. The results shown are representative of three similar experiments obtained with cells derived from different donors.
