The molecular basis for adhesion-mediated suppression of reactive oxygen species generation by human neutrophils
J. Clin. Invest. Tieming Zhao, et al. 112:1732 doi:10.1172/JCI19108 [
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Figure 4Constitutively active Rac2 restores adhesion-mediated inhibition of NADPH oxidase activity in neutrophils and a reconstituted cell-free system. (
a) Nontreated neutrophils, neutrophils expressing WT Rac2, or cells expressing constitutively active Rac2(V12), as described in Methods, were tested for H
2O
2 production in response to 1 × 10
–7 M fMLF under suspension and adherent conditions. Results shown are representative of three independent experiments using cells from different donors. (
b) Human neutrophil membrane and cytosol fractions were prepared from suspended or adherent cells at early (0 minutes and 5 minutes, respectively) times after stimulation with 1 × 10
–7 M fMLF, as described in Methods. Superoxide anion formation was determined with various mixtures of membranes and cytosols, as indicated. Results shown are the mean ± SD of three independent experiments. ΔA, change in absorbance at 550 nm.
