The anaphylatoxin C3a downregulates the Th2 response to epicutaneously introduced antigen
J. Clin. Invest. Seiji Kawamoto, et al. 114:399 doi:10.1172/JCI19082 [Go to this article.]

Figure 9
C3a inhibition of IL-4 production is mediated by IL-12. (A) C3a induces IL-12 p70 secretion by BALB/c splenic DCs. (B) Immunodepletion of IL-12 abrogates the IL-4–inhibitory activity of C3a-stimulated DC supernatants. Supernatant of C3a-stimulated DCs were first depleted of C3a, then subjected to immunoaffinity chromatography using protein G columns immobilized with anti–mouse IL-12 p40, anti-IL-12 p35 mAb, or isotype controls, and then tested for inhibition of IL-4 production as detailed in Figure 8B. (C) Impaired IL-4–inhibitory activity of C3a-stimulated DC supernatant from IL-12–deficient mice. C3a-stimulated splenic DC supernatants from IL-12 p40^–/–, p35^–/–, or WT C57BL/6 mice were tested for inhibition of IL-4 production as detailed in Figure 8B. Columns and error bars represent mean ± SEM of three experiments. *P < 0.05.