Targeted ablation of Fgf23 demonstrates an essential physiological role of FGF23 in phosphate and vitamin D metabolism
J. Clin. Invest. Takashi Shimada, et al. 113:561 doi:10.1172/JCI19081 [Go to this article.]

Figure 4
Key molecules for serum phosphate and 1,25(OH)₂D levels. (a) Northern blot analysis of renal 1α-OHase and 24-OHase mRNAs. The blots were reprobed with a GAPDH fragment to confirm integrity of the electrophoresed RNAs. (b) TmP/GFR. Mice (6 weeks old) were reared in metabolic cages to collect urine samples for 24 hours. The results represent mean ± SEM. WT, n = 6; heterozygotes, n = 6; homozygotes, n = 6. **P < 0.01, evaluated by Dunnett’s method. (c) Immunohistochemistry of renal NaP_i-2a protein at 6 weeks of age.