Enhancement of MEK/ERK signaling promotes glucocorticoid resistance in CD4⁺ T cells
J. Clin. Invest. Daphne C. Tsitoura, et al. 113:619 doi:10.1172/JCI18975 [Go to this article.]

Figure 4
Costimulation restores the inhibition of AP-1 activation by Dex. Nuclear extracts were prepared from Dex-treated and control naive CD4+ T cells stimulated with anti-CD3, anti-CD3 plus IL-2, or anti-CD3 plus anti-CD28 and analyzed by electrophoretic mobility shift assay (EMSA) using 32P-labeled oligonucleotide probes corresponding to (a) AP-1, (b) NF-AT, or (c) NF-κB DNA binding sites. As a control, the competition (Comp) for DNA binding with excess oligonucleotide was assessed. Results from a representative experiment are shown.