Hepatoprotection by the farnesoid X receptor agonist GW4064 in rat models of intra- and extrahepatic cholestasis
J. Clin. Invest. Yaping Liu, et al. 112:1678 doi:10.1172/JCI18945 [
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Figure 3Liver gene expression profile in ANIT model of cholestasis. Total RNA was isolated from rat liver or primary human hepatocytes, and gene expression was measured using RTQ-PCR. (
a) Gene expression profile in the liver of rats with ANIT-induced cholestasis. White bars, vehicle/vehicle; black bars, vehicle/ANIT; dark gray bars, GW4064/ANIT; light gray bars, TUDCA/ANIT. Statistically significant differences from the vehicle/vehicle group are indicated (
#P < 0.05). Statistically significant differences from the vehicle/ANIT group are also indicated (*
P < 0.05). (
c) Induction of
MDR2,
BSEP, and
SHP in the liver of the normal rat treated once daily for 4 days with corn oil vehicle (white bars) or GW4064, 30 mg/kg intraperitoneally (dark gray bars). Statistically significant inductions are indicated (*
P < 0.05). (
d) Induction of
MDR3,
BSEP, and
SHP in primary human hepatocytes treated 12 hours with 0.1% DMSO as vehicle (white bars) or 1 μM GW4064 (dark gray bars). Statistically significant inductions are indicated (*
P < 0.05).
