Hepatoprotection by the farnesoid X receptor agonist GW4064 in rat models of intra- and extrahepatic cholestasis
J. Clin. Invest. Yaping Liu, et al. 112:1678 doi:10.1172/JCI18945 [Go to this article.]

Figure 2
Protection against ANIT-induced necrosis by GW4064. Rats (n = 6–8) were treated once daily with vehicle (Veh), GW4064, or TUDCA. On the second treatment day the rats received a single dose of ANIT or vehicle. Livers were taken for histological analysis 4 hours after the final dose. The panels show representative H&E-stained liver sections from each treatment group at ×400 magnification. (a) Vehicle/vehicle–treated rat showing normal liver histology. (b) Vehicle/ANIT–treated rat showing a large area of parenchymal necrosis (filled arrow) with inflammatory cell infiltration (open arrow). (c) GW4064/ANIT–treated rat showing inflammatory cell infiltration (open arrow) around the bile duct, but no necrosis. (d) TUDCA/ANIT–treated rat showing parenchymal necrosis (filled arrow) with inflammatory cell infiltration (open arrow).