Inactivation of fatty acid transport protein 1 prevents fat-induced insulin resistance in skeletal muscle
J. Clin. Invest. Jason K. Kim, et al. 113:756 doi:10.1172/JCI18917 [Go to this article.]

Figure 1
Generation of FATP1 KO mice. (A) Map of the FATP1 genomic locus and the targeting constructs. The 5′ and 3′ probes that were used to verify the homologous recombination event are indicated. (B) Southern blot using genomic DNA from the tail of F₂ animals digested with EcoRI and SacI and hybridized with the 5′ and 3′ probes, respectively. The size of KO and WT alleles is indicated. (C) DNA products obtained by PCR amplification using the genotyping primers described in Methods and genomic DNA from the tail of F₂ animals as a template. nt, nucleotide. (D and E) FATP1 mRNA levels in white adipose tissue (WAT) and skeletal muscle of KO and WT animals.