TGF-β switches from tumor suppressor to prometastatic factor in a model of breast cancer progression
J. Clin. Invest. Binwu Tang, et al. 112:1116 doi:10.1172/JCI18899 [
Go to this article.]
Figure 5Decreased TGF-β responsiveness does not affect primary tumorigenesis but suppresses metastasis in the high-grade breast carcinoma line M-IV. (
a) Tumor growth kinetics in vivo. Nude mice were inoculated subcutaneously on each hind flank with retrovirally transduced M-IV cells (2 × 10
5 cells/site). For M-IV CON,
n = 11 sites injected; M-IV DNR,
n = 11. Untransduced parental M-IV cells gave essential identical results to M-IV CON (
n = 4, not shown). (
b) Lung metastases. Metastatic efficiency was determined by quantitation of histologically detectable lung metastases 8 weeks after injection of 10
6 retrovirally transduced cells into the tail vein of nude mice. Results are the mean ± SD for
n = 5 (M-IV CON) and
n = 10 (M-IV DNR). CON, cells transduced with pLPCX; DNR, cells transduced with pLPC-DNR.
