Diabetes and exocrine pancreatic insufficiency in E2F1/E2F2 double-mutant mice
J. Clin. Invest. Ainhoa Iglesias, et al. 113:1398 doi:10.1172/JCI18879 [
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Figure 6Increased proliferation and apoptosis in pancreas of E2F1/E2F2 compound-mutant mice. In vivo BrdU labeling (as described in Methods) identified pancreatic cells in S phase (appearing brown) in pancreata from 15-day-old mice. As shown, young DKO mice have an increased number of cells in S phase in the pancreas in vivo. Arrowheads in lower left panel indicate BrdU-positive nuclei. The presence of the expected labeling of intestinal crypt cells with BrdU in both genotypes (inset) serves as an internal control for the injection and incorporation of the tracer. Cell death was detected by TUNEL labeling in 15-day-old mice. Arrowheads in lower right panel denote apoptotic nuclei (appearing brown) detected by the TUNEL assay. Virtually no BrdU-positive or TUNEL-positive cells were detected in 15-day-old WT pancreata. The presence of the expected staining of thymocytes with TUNEL in both genotypes (inset) serves as an internal control for the experiment.
