Chronic phospholamban inhibition prevents progressive cardiac dysfunction and pathological remodeling after infarction in rats
J. Clin. Invest. Yoshitaka Iwanaga, et al. 113:727 doi:10.1172/JCI18716 [Go to this article.]

Figure 1
Enhanced shortening and SR calcium cycling in isolated adult rat cardiomyocytes treated with S16EPLN. Ventricular cardiomyocytes were isolated from adult Wistar rats according to the protocol described by Zho et al. (30). Cells were transfected with adenovirus vectors expressing LacZ (Adeno-LacZ) or S16EPLN (Adeno-S16EPLN) at a multiplicity of infection of 100 and cultured for 36 hours. Nearly 100% efficiency of transfection was confirmed by β-gal staining of Adeno-LacZ–treated cells. Thereafter, both Adeno-LacZ–treated cells and Adeno-S16EPLN–treated cells were loaded with fura-2/AM, and cell shortening and changes in intracellular-calcium concentration were monitored. (A and B) Representative tracings of cell length and fura-2 340/380 ratio, an index of calcium concentration. (C–E) Indices of cell shortening (percentage fractional shortening [% FS] and –dL/dt) and relaxation (+dL/dt). (F and G) The averaged peak amplitude of an index of calcium transient (F) and the averaged decay time constant of the descending limb of calcium transient (G). Data represent mean ± SE and are accumulated from five independent experiments from five animals. A total of 40 cells were measured for each treatment group. *P < 0.05 between groups. L, length.