TLR4 signaling induces TLR2 expression in endothelial cells via neutrophil NADPH oxidase
J. Clin. Invest. Jie Fan, et al. 112:1234 doi:10.1172/JCI18696 [Go to this article.]

Figure 5
Increased TLR2 expression results in augmented and stable ICAM-1 expression. (a) The results show the effects of sequential challenges of LPS and PGN, the ligands for TLR4 and TLR2, respectively, on ICAM-1 expression in lungs. In WT mice, LPS (10 μg/10 g body wt), PGN (10 μg/10 g body wt), LPS plus PGN, or saline (SAL) was injected intraperitoneally at time 0, and 2 hours later, a dose of either LPS (10 μg/10 g body wt), PGN (10 μg/10 g body wt), or saline was injected. Lung tissue was harvested at the times indicated, and ICAM-1 protein was detected using Western blotting. (b) WT or TLR2^–/– mice were injected with E. coli (1 × 10⁵ cells/10 g body wt, intraperitoneally) and ICAM-1 expression in the lung was measured 4 hours later by Western blotting. The data are representative of three independent studies.