TLR4 signaling induces TLR2 expression in endothelial cells via neutrophil NADPH oxidase
J. Clin. Invest. Jie Fan, et al. 112:1234 doi:10.1172/JCI18696 [Go to this article.]

Figure 1
LPS upregulates TLR2 expression in lungs and endothelial cells through TLR4 and MyD88 signaling. (a and b) Mice were injected intraperitoneally with LPS (10 μg/10 g body wt) and whole lungs were harvested at the times indicated after LPS injection. Data are representative of three independent studies. (a) RT-PCR for TLR2 mRNA expression in the lungs. GAPDH mRNA is identified as a control for RNA loading. (b) Western blot for TLR2 protein in lungs. Corresponding actin is shown as evidence of comparable loading between lanes. (c and d) MLVECs were incubated with LPS (1 μg/ml) for 2 hours, TLR2 mRNA was detected using RT-PCR (c), and TLR2 protein level was measured by Western blotting (d). The data are representative of three independent studies.