Modulation of the molecular composition of large conductance, Ca²⁺ activated K⁺ channels in vascular smooth muscle during hypertension
J. Clin. Invest. Gregory C. Amberg, et al. 112:717 doi:10.1172/JCI18684 [Go to this article.]

Figure 3
Functional and pharmacologic properties of single BK channels indicate decreased β1 subunit function in HT myocytes. (a) Ca²⁺sensitivity of BK channels in inside-out patches (HP = –40 mV) from NT and HT myocytes. Shown to the left are representative single BK channel records taken from NT and HT patches in the presence of 1 or 10 μM Ca²⁺. The bar plot to the right shows the mean ± SEM P_o of BK channels in NT and HT patches at three Ca²⁺ concentrations. (b) Open-time analysis of BK channels in inside-out patches from NT and HT myocytes. Shown to the left are representative single BK channel records taken from NT and HT patches at +40 mV in the presence of 1 μM Ca²⁺. The open-time histograms of these BK channels from NT and HT myocytes are shown in the center. Histograms were fitted with a single exponential function. The bar plot to the right shows the mean ± SEM τ_open of BK channels in NT and HT cells. (c) Tam (1 μM) sensitivity of BK channels in inside-out patches (HP = +40 mV; 100 nM free Ca²⁺) from NT and HT myocytes. Shown to the left are representative single BK channel records taken from NT and NT before and after the application of Tam. The bar plot to the right shows the mean ± SEM fold change in the P_o of BK channels in NT and HT cells after the application of Tam. (d) Number of BK channels per patch. Dashed lines indicate open channels. o, open channel; c, closed channel. *P < 0.05.