A role for surface lymphotoxin in experimental autoimmune encephalomyelitis independent of LIGHT
J. Clin. Invest. Jennifer L. Gommerman, et al. 112:755 doi:10.1172/JCI18648 [Go to this article.]

Figure 2
CD4⁺ T cells from LTβR-Ig–treated rats are hyporesponsive ex vivo. (a) Pooled CD4⁺ T cells were isolated at D10 from rats in the EAE model (n = 5), stimulated in vitro with irradiated splenocytes, and indicated concentrations of MBP-peptide and proliferation measured by ³H-thymidine incorporation. Cells were derived from rats treated in vivo with control huIgG (diamonds), LTβR-Ig (circles), or from naive, untreated rats as controls (squares). (b) Supernatants from cultures in a (0 and 2.5 μg/ml MBP-peptide, white and gray bars, respectively) were collected at 72 hours and measured by ELISA for IFN-γ content. The experiment was repeated three times with similar results and also performed at day 7 with similar results.