Impaired pancreatic growth, β cell mass, and β cell function in E2F1 ^–/– mice
J. Clin. Invest. Lluis Fajas, et al. 113:1288 doi:10.1172/JCI18555 [Go to this article.]

Figure 4
Pancreatic size and β cell area. (A) H&E staining of pancreatic sections of E2F1^_/_ or E2F1^+/+ 4-week-old mice. Pancreatic islets are indicated by arrowheads. (B) Pancreatic islet number in E2F1^_/_ and E2F1^+/+ 4-week-old mice. Summed islet numbers obtained from examining every fifth section of 20-∝m thickness from a whole pancreas (left scale). The numbers were relative to pancreas weight to determine the islet/pancreas ratio (right scale). (C) Endocrine islet area calculated as arbitrary units (arb. units) from micrographs of pancreatic sections of E2F1^+/+ or E2F1^_/_ 4-week-old mice using the Canvas 7 software. The right scale represents the islet area relative to pancreas weight. (D) H&E staining of pancreatic sections of E2F1^_/_ and E2F1^+/+ mice. Quantification of the relative (rel.) cell size of exocrine pancreatic cells of 2-week-old E2F1^_/_ and E2F1^+/+ mice. (E) Immunofluorescence analysis of pancreatic sections showing expression of E2F1 in ducts and β cells (green), which are also positive for insulin (red). Nuclei are stained with the Hoechst reagent. *Statistically significant differences (ANOVA, P < 0.005).