Endothelial and nonendothelial sources of PDGF-B regulate pericyte recruitment and influence vascular pattern formation in tumors
J. Clin. Invest. Alexandra Abramsson, et al. 112:1142 doi:10.1172/JCI18549 [Go to this article.]

Figure 2
Quantitative analyses of tumor vessels in T241 tumors grown on WT C57BL6 mice (T241/WT), T241 tumors grown on pdgf-b^ret/ret mice (T241/ret), T241-B tumors grown on WT C57BL6 mice (T241-B/WT), and T241-B tumors grown on pdgf-b^ret/ret mice (T241-B/ret). (a) Mean vessel diameter. T241/WT, 11.7 ± 9; T241/ret, 26.8 ± 16; T241-B/WT, 14.5 ± 5.5; T241-B/ret, 14.9 ± 6.5 μm. (b) Scatter plot of data in a. (c–f) Analysis of pericyte density and coverage. (c) Example of collected data. (d) Pericyte coverage illustrated as NG2–Pecam-1 overlapping area, expressed as a percentage of total Pecam-1–stained area. (e) Scatter plot of the data in d. (f) Pericyte density illustrated as NG2 area as a percentage of total Pecam-1 area. (g–i) Pericyte “detachment” from the endothelial cells expressed as the distance (in μm) from the endothelium to the most distal part of a pericyte. (g) Example of collected data. (h) Mean distance (μm) of mural cell association with tumor endothelium. (i) Scatter plot of individual NG2-positive pericytes. *P < 0.05.