Alteration of the 4-sphingenine scaffolds of ceramides in keratinocyte-specific Arnt-deficient mice affects skin barrier function
J. Clin. Invest. Satoshi Takagi, et al. 112:1372 doi:10.1172/JCI18513 [Go to this article.]

Figure 7
HPLC/ion-trap mass spectroscopy of SC ceramides in WT and Arnt-null mice. (a) Total ion current (TIC) and ion chromatograms of the most intense signals of Cer(EOS), Cer(NS), Cer(NP), and Cer(AS) in the positive-ion mode. Where the m/z values of these signals were different from each other in WT and Arnt-null mice, the sum of ion chromatograms of both m/z values, except WT Cer(AS), is presented. Ion chromatograms of ceramides containing 4-sphingenine are presented as the sum of the most intense [M + H]⁺ ions and their dehydrated ions. (b) Ratios of peak areas of Cer(EOS), Cer(NP), and Cer(AS) to those of Cer(NS) in WT (gray bars)and Arnt-null (black bars) SC. To generate a reconstituted chromatogram for each ceramide species, ceramide-related signals with intensities of greater than 5% of that of the most intense peak were used. Cer(AS) was divided into Cer(AS)_s, with shorter-chain acyl moieties (mainly C16:0 and C18:0), and Cer(AS)_l, with longer-chain acyl moieties (mainly C24:0–C26:0).