Alteration of the 4-sphingenine scaffolds of ceramides in keratinocyte-specific Arnt-deficient mice affects skin barrier function
J. Clin. Invest. Satoshi Takagi, et al. 112:1372 doi:10.1172/JCI18513 [Go to this article.]

Figure 4
EM analysis. EM images of SC from WT (a, c, and e) and Arnt-null mice (b, d, and f). SC from Arnt-null mice is tightly packed, and abnormal vesicles are present (compare a and b). In the granular-layer keratinocytes, lamellar bodies with disk-like contents were observed and excreted normally into the intercellular space of the SC (c and d). Arrowheads indicate lamellar bodies. The intercellular lamellar structure appeared normal (e and f). Arrows indicate the intercellular lamellar structure. Bars: 1 μm (a and b), 0.2 μm (c and d), and 0.1 μm (e and f). (g) A closer comparison of SC lamellae in WT and Arnt-null mice. Their images were obtained by scanning of negatives taken at a primary magnification of ×40,000 at 12-bit gray-scale resolution and were analyzed with NIH ImageJ software (48).