Activation of diverse repertoires of autoreactive T cells enhances the loss of anti-dsDNA B cell tolerance
J. Clin. Invest. Brian W. Busser, et al. 112:1361 doi:10.1172/JCI18310 [Go to this article.]

Figure 5
Limited T cell repertoires are activated but fail to enter the B cell follicle. (a) Donor Thy1.2⁺ CD4 cells were identified 7 days after induction of cGVHD in B6.PL/3H9.KI mice by staining with Thy1.2 and CD4. Gated populations of the contour plots represent the expansion in percentage of transferred CD4⁺ T cells for the indicated cell population. The total number of recovered Thy1.2⁺ CD4⁺ splenocytes is indicated above the plot. Representative results from four experiments are shown. (b) Donor CD4⁺ cells were localized by staining spleen sections with Ab’s against Thy1.2 (brown) and B220 (blue). The few B6 CD4⁺ T cells detected by IHC were scattered throughout the T cell area. Activation of the class II–reactive T cell repertoires resulted in the majority of the cells migrating to T-B interface. The diverse T cell repertoires (K14 and bm12) contained T cells that were also evident in the B cell follicle; however, activation of the limited T cell repertoires (2-2-3/K14 and H2-DM^–/–) did not result in the presence of transferred Thy1.2⁺ T cells in the follicle. Representative results of four experiments are shown.