Activation of diverse repertoires of autoreactive T cells enhances the loss of anti-dsDNA B cell tolerance
J. Clin. Invest. Brian W. Busser, et al. 112:1361 doi:10.1172/JCI18310 [Go to this article.]

Figure 3
Introduction of an anti-dsDNA heavy chain does not alter the behavior of the class II–reactive T cell repertoires. (a) cGVHD was induced by injecting B6/3H9.KI mice with 10 × 10⁶ of the indicated MHC class II–reactive CD4⁺ T cell repertoires. Serum samples were taken and tested for IgG anti-chromatin levels by ELISA. Limited T cell repertoires (2-2-3/K14 and H2-DM^–/–) failed to stimulate a significant increase in the titers of IgG anti-chromatin autoantibodies as compared with B6 negative control at all time points tested (P > 0.05); the diverse T cell repertoires induced a significant increase at all time points (P < 0.05). The diverse T cell repertoires, K14 and bm12, induced a comparable (P > 0.1) elevation of IgG anti-chromatin autoantibodies. Each group includes at least eight experimental mice. (b) Serum samples from B6/3H9.KI mice that received the indicated MHC class II–reactive CD4⁺ T cell repertoires were tested for IgG anti-dsDNA levels by ELISA. Similar to the failure to induce IgG anti-chromatin autoantibodies, limited T cell repertories (2-2-3/K14 and H2-DM^–/–) failed to stimulate a significant increase in IgG anti-dsDNA Ab production as compared with transfer of B6 CD4⁺ T cells at all time points tested (P > 0.05), whereas activation of the diverse T cell repertoires (K14 and bm12) induced a significant increase at all time points (P < 0.05). Each group contains at least eight experimental mice.