Identification of App1 as a regulator of phagocytosis and virulence of Cryptococcus neoformans
J. Clin. Invest. Chiara Luberto, et al. 112:1080 doi:10.1172/JCI18309 [Go to this article.]

Figure 2
(a) DD-RT-PCR in WT and GAL7:IPC1 10 strains using dT-A, -C, and -G with AP1 and AP2 primers. The number 1 denotes WT-glucose; 2, GAL7:IPC1-glucose; 3, WT-galactose; and 4, GAL7:IPC1-galactose. The black arrow indicates the App1 band, which is downregulated when Ipc1 is downregulated (no. 2) and upregulated when Ipc1 is upregulated (no. 4), with no significant changes in WT-glucose (no. 1) or WT-galactose (no. 3). (b) Ipc1 activity, RT-PCR, and Western blot of App1 in C. neoformans WT H99 and GAL7:IPC1 strains grown on glucose and galactose medium. APP1 expression is significantly regulated by Ipc1 modulation. Actin gene was used as a control. Two independent GAL7:IPC1 strains were used for RT-PCR (nos. 10 and 13).