Hyper-IgM syndrome type 4 with a B lymphocyte–intrinsic selective deficiency in Ig class-switch recombination
J. Clin. Invest. Kohsuke Imai, et al. 112:136 doi:10.1172/JCI18161 [Go to this article.]

Figure 3
Detection of RNA transcripts of CSR-related genes. (a) RNA transcripts of the CD19 (as control), AID, germ-line (εGLTs), circular (εCTs), and functional (εFTs) transcripts for IgE, and of the functional transcripts for IgG (γFTs) were detected by RT-PCR after stimulation with sCD40L+rIL-4 for 5 days in control subjects (C) and in HIGM4 patients (P1, P2, P5, P7, P8, P11, P15). (b) CD19 transcripts and εGLTs levels were analyzed using different quantities of cDNA (1:1, 1:10, 1:100, and 1:1,000 dilution) in RT-PCR for selected cases (P1, P5, P7, P15).