Chemokine-mediated recruitment of NK cells is a critical host defense mechanism in invasive aspergillosis
J. Clin. Invest. Brad E. Morrison, et al. 112:1862 doi:10.1172/JCI18125 [Go to this article.]

Figure 5
Role of MCP-1/CCL2 in in vivo recruitment of NK cells to the lungs in invasive aspergillosis. Cultured NK cells were left unlabeled or labeled with the vital cytoplasmic fluorochrome, CFSE, and transferred to neutrophil-depleted mice before intratracheal challenge with A. fumigatus conidia or vehicle. Infected mice were treated with PBS or neutralizing anti–MCP-1/CCL2 Ab, and lungs were examined for the presence of CFSE-labeled cells after 1 day. (a) A representative scatter diagram of total lung cells, demonstrating the gate used in b. (b) Representative scatter diagrams from each group, gated on lung CD3^– NK1.1⁺ cells in a. The upper-right quadrants contain transferred CFSE⁺ NK cell populations used for statistical comparisons in c. (c) Mean ± SEM of the number of lung CFSE⁺ CD3^– NK cells in each group (n = 3–5 per group). *P < 0.05 compared with uninfected mice given labeled cells; **P < 0.05 compared with infected mice with PMN depletion.