Critical role of IL-10 in the induction of low zone tolerance to contact allergens
J. Clin. Invest. Marcus Maurer, et al. 112:432 doi:10.1172/JCI18106 [Go to this article.]

Figure 4
IL-10–producing regulatory CD4⁺ T cells are required for the induction of LZT. CD4⁺ and CD8⁺ T cells were separated from LNCs obtained from naive IL-10^+/+ and IL-10^–/– mice as described above and adoptively transferred into T cell–deficient Rag1^–/– mice (2 × 10⁷ CD4⁺ and 1 × 10⁷ CD8⁺ T cells). Subsequently, the mice were treated repeatedly with low doses of contact allergen (TNCB) to induce LZT and were then sensitized and challenged. IL-10^–/– and IL-10^+/+ served as control mice for CHS and LZT induction. (a) Ear thickness (percentage ± SEM) and (b) proliferation (cpm in % of sensitized control mice) of LNCs after hapten-specific restimulation in vitro pooled from six or more mice per treatment group from one of two independent experiments with similar results was shown.