Critical role of IL-10 in the induction of low zone tolerance to contact allergens
J. Clin. Invest. Marcus Maurer, et al. 112:432 doi:10.1172/JCI18106 [Go to this article.]

Figure 3
Low zone tolerization upregulates IL-10 production in LN CD4⁺ T cells. (a) LZT quantified by assessing contact hypersensitivity reaction (ear swelling) after TNCB challenge in Kit^W/Kit^W-v mice, which are genetically deficient for IL-10–producing MCs, and Kit^+/+ mice that were treated repeatedly with low doses of contact allergen (TNCB) to induce LZT and then were sensitized to TNCB. (b) At various time points after application of sensitizing or tolerizing doses of TNCB or AOO alone, ears were excised, and supernatants of the tissue extracts were prepared as described in Methods. Amounts of IL-10 were measured by ELISA, and the results were normalized to amounts of total protein. Results are presented as picograms per milligram of protein of the tissue extract. ^#P < 0.001. (c and d) IL-10 release from T cells (CD4⁺ or CD8⁺) and non–T cells isolated from LNCs obtained from control (–) or tolerized (+) mice after induction of LZT was measured by ELISA (c) or by ELISPOT (d) after hapten-specific restimulation in vitro.