Increased T cell reactivity to amyloid β protein in older humans and patients with Alzheimer disease
J. Clin. Invest. Alon Monsonego, et al. 112:415 doi:10.1172/JCI18104 [Go to this article.]

Figure 4
Activated Aβ-reactive T cells exhibit Th1, Th2, and Th0 phenotypes after stimulation with autologous PBMCs and Aβ peptides. Aβ-reactive T cells were established from a secondary stimulation assay as described in Methods. Resting cells were cultured with autologous PBMCs alone or stimulated in the presence of Aβ1–42, Aβ15–42, or Aβ1–28 and harvested after 30 hours. Cells were stained with Cy-chrome–labeled anti-CD4 and FITC-labeled anti-CD69 (a) followed by intracellular staining with phycoerythrin-labeled anti–IFN-γ, –IL-13, –IL-5, –IL-10, and –IL-12 (b). Cells also were stained with Cy-chrome–labeled anti-CD4 followed by intracellular staining with FITC-labeled anti–IFN-γ and phycoerythrin-labeled anti–IL-13 or anti–IL-5 (c). Positive cells were not observed when intracellular staining was performed with isotype controls (data not shown). For (b) and (c), analysis was gated on CD4⁺ cells. No Ag., no antigen.