Increased T cell reactivity to amyloid β protein in older humans and patients with Alzheimer disease
J. Clin. Invest. Alon Monsonego, et al. 112:415 doi:10.1172/JCI18104 [
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Figure 3Epitope specificity and cytokine profile of Aβ-reactive T cell lines. Split-well assays were performed as described in Methods. To generate Aβ-reactive T cell lines, positive wells were restimulated in the presence of irradiated PBMCs and Aβ and then maintained with 10 U/ml IL-2. To determine Aβ and epitope specificity, T cell lines were stimulated with Aβ1–42 and two overlapping peptides, Aβ1–28 or Aβ15–42, followed by their stimulation with nested peptides of the Aβ15–42 region. To measure Aβ-induced cytokine production, supernatants were collected 48 hours after stimulation and examined by ELISA. T cell proliferation of three representative lines is shown: (
a–
c) induced by Aβ1–42 (
a and
c) Aβ15–42, and (
b) Aβ1–28. T cell proliferation was also induced by (
d) Aβ16–30 (
e) Aβ19–33, and (
f) Aβ28–42. Aβ-induced secretion of IFN-γ and IL-13 is shown in
g through
l.
