Increased T cell reactivity to amyloid β protein in older humans and patients with Alzheimer disease
J. Clin. Invest. Alon Monsonego, et al. 112:415 doi:10.1172/JCI18104 [
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Figure 1Aβ1–42 is more immunogenic than Aβ1–40. PBMCs were isolated from nine subjects (three adults, three older healthy subjects, and three patients with clinically mild or moderate AD) and cultured in U-bottom 96-well plates in the presence of human Aβ1–40 or Aβ1–42 (30 wells per peptide) as described in Methods. Cells were then restimulated at day 10 in the presence or absence of the peptide used initially. T cell proliferation was measured by [
3H]thymidine incorporation 72 hours after the secondary stimulation. The percentage of positive wells was obtained according to the number of wells (out of 30) with SIs (cpm in the presence of antigen divided by cpm in the absence of antigen) of at least 2.5 and a Δcpm of at least 2,000.
