Loss of pain perception in diabetes is dependent on a receptor of the immunoglobulin superfamily
J. Clin. Invest. Angelika Bierhaus, et al. 114:1741 doi:10.1172/JCI18058 [Go to this article.]

Figure 1
Localization of CML, RAGE, activated NF-κBp65, and IL-6 antigens in sural nerve biopsies from patients with diabetes mellitus. (A) Representative immunohistochemical staining of a sural nerve biopsy from a 51-year-old female patient with type 1 diabetes mellitus: CML (first column), RAGE (second column), activated NF-κBp65 (third column), and IL-6 antigen (fourth column) in epineurial vessels (top), perineurium (middle), and endoneurial vessels (bottom). Positivity for the respective antigen is indicated by intense red staining. The faint blue background color is due to counterstaining with hemalaun, indicating the integrity of the tissue investigated. Scale bars: 100 μm. (B) Quantification of staining intensities of epineurial vessels (EP), perineurium (P), and endoneurial vessels (EN) in nondiabetic (black bars, n = 8) and diabetic (gray bars, n = 10) patients. Data are means ± SD; single asterisks denote P values less than 0.05, which we considered to be statistically significant; double asterisks denote P values less than 0.005, which we considered to be highly statistically significant. (C) Comparison of the staining intensity for the RAGE ligand CML (left) and the RAGE ligand S100A8/A9 (right) in sural nerve biopsies from a 56-year-old male patient with CMT-I (top) and a 66-year-old male patient with type 2 diabetes (bottom). Representative epineurial vessels are shown. Magnification, ×400.