Pyk2 activation is integral to acid stimulation of sodium/hydrogen exchanger 3
J. Clin. Invest. Shaoying Li, et al. 114:1782 doi:10.1172/JCI18046 [
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Figure 9Pyk2 is required for acid activation of c-Src. OKP cells transiently transfected with wild-type or dominant-negative (DN)
pyk2 and wild-type
c-Src were grown to confluence, rendered quiescent, and exposed to control (pH 7.4) or acid (pH 6.8) medium for 90 seconds; c-Src was immunoprecipitated as described in Methods. c-Src kinase activity was assayed using acid-denatured enolase as the substrate. (
A) Typical autoradiograph (top) and Western blot (bottom). (
B) Summary of the data, which are normalized for c-Src abundance and plotted as percentage of control (pH 7.4);
n = 6 for studies done with wild-type Pyk2 and
n = 4 for studies done with the dominant-negative Pyk2. c-Src kinase activity was increased in 6 of 6 experiments in the presence of the wild-type Pyk2 and in 1 of 4 experiments in the presence of the dominant-negative Pyk2.
