Pyk2 activation is integral to acid stimulation of sodium/hydrogen exchanger 3
J. Clin. Invest. Shaoying Li, et al. 114:1782 doi:10.1172/JCI18046 [
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Figure 8Acid incubation increases Pyk2 and c-Src coimmunoprecipitation. Representative blots. OKP cells transiently transfected with
pyk2 and
c-Src were grown to confluence, rendered quiescent, and exposed to control (C) (pH 7.4) or acid (A) (pH 6.8) medium for the indicated time; Pyk2 or v-Src was immunoprecipated (IP) as indicated. The immunoprecipitates were divided and immunoblotting (IB) performed using anti-Pyk2 and anti-c-Src antibodies as indicated;
n = 3 for each group.
