Pyk2 activation is integral to acid stimulation of sodium/hydrogen exchanger 3
J. Clin. Invest. Shaoying Li, et al. 114:1782 doi:10.1172/JCI18046 [Go to this article.]

Figure 7
siRNA_pyk2 prevents NHE3 activation by acid. OKP cells were transiently transfected with 1.33 μg siRNA_pyk2 (pyk2 siRNA) per well for 24 hours (at which time they were confluent), rendered quiescent for 18 hours, and exposed to control (pH 7.4) or acid (pH 6.8) medium for 6 hours; then Na/H antiporter activity was assayed. Data are plotted as dpHi/dt, pH units/min. Controls were cells exposed to transfection reagent without siRNA (No siRNA) or cells transfected with a siRNA against a nonmammalian protein (GL2 siRNA); n = 7 (No siRNA), n = 6 (GL2 siRNA), and n = 10 (pyk2 siRNA). * P < 0.05 vs. pH 7.4.