Pyk2 activation is integral to acid stimulation of sodium/hydrogen exchanger 3
J. Clin. Invest. Shaoying Li, et al. 114:1782 doi:10.1172/JCI18046 [Go to this article.]

Figure 6
siRNA_pyk2 decreases Pyk2 protein abundance in OKP cells. Typical blots. (A) Cells were transfected with 1.33 μg/well siRNA_pyk2 or siRNA_GL2 (GL2 siRNA) or transfection reagent only (No siRNA) for 24 hours (at which time they were confluent), rendered quiescent for 24 hours, and harvested. FAK protein abundance was measured for comparison. (B) Cells were transfected with 1.33 μg/well siRNA_pyk2 for 24 hours (at which time they were confluent) and rendered quiescent. The length of quiescence varied from 0 to 48 hours, so that cells were harvested 24, 48, or 72 hours after the beginning of transfection. (C) Cells were transfected with the indicated amount of siRNA_pyk2 for 24 hours (at which time they were confluent), rendered quiescent for 24 hours, and harvested.