Pyk2 activation is integral to acid stimulation of sodium/hydrogen exchanger 3
J. Clin. Invest. Shaoying Li, et al. 114:1782 doi:10.1172/JCI18046 [
Go to this article.]
Figure 5Dominant-negative Pyk2 prevents NHE3 activation by acid. OKP cells were transiently transfected with 1 μg of the indicated DNA for 5 hours, grown to confluence over the next 19 hours, rendered quiescent for 24 hours, and exposed to control (pH 7.4) or acid (pH 6.8) media for 6 hours; Na/H antiporter activity was then assayed as the rate of Na-dependent increase in cell pH. Data are plotted as dpHi/dt, pH units/min;
n = 6 (wild-type cells),
n = 19 (vector transfected),
n = 19 (wild-type Pyk2 transfected), and
n = 17 (dominant-negative [DN] Pyk2
K457A transfected). *
P < 0.01 vs. pH 7.4.
