Pyk2 activation is integral to acid stimulation of sodium/hydrogen exchanger 3
J. Clin. Invest. Shaoying Li, et al. 114:1782 doi:10.1172/JCI18046 [Go to this article.]

Figure 2
Acid activates Pyk2 in a cell-free system. Wild-type OKP cells were grown to confluence and rendered quiescent for 48 hours. Pyk2 was immunoprecipitated as described in Methods and exposed to buffers at the indicated pH for 5 minutes; kinase activity was assayed as the ability of Pyk2 to phosphorylate poly(Glu-Tyr)^4:1. (A) Typical autoradiograph (top) and Western blot (bottom). (B) Summary of the data, which are normalized for Pyk2 abundance and plotted as a percentage of control (pH 7.4); n = 9 for each pH. Pyk2 kinase activity was increased in 8 of 9 experiments at pH 7.2, 7.0, and 6.8.