BAFF selectively enhances the survival of plasmablasts generated from human memory B cells
J. Clin. Invest. Danielle T. Avery, et al. 112:286 doi:10.1172/JCI18025 [Go to this article.]

Figure 2
BAFF enhances survival but does not affect proliferation of human B cells. (a) Total splenic B cells were cultured in medium alone (squares) or with BAFF (circles; 2.5 μg/ml) and the number of viable cells determined after 2 and 4 days. (b) Total, naive, or memory B cells were cultured with media alone (black bars), BAFF (white bars), or CD40L (gray bars), and the number of surviving cells was quantitated after 4 days. Each point represents the mean ± SD of duplicate samples and is representative of three different experiments. Error bars are shown for all graphs; however, they are not always visible. For the experiment shown, BAFF increased the survival of total, naive, and memory B cells 1.95-, 1.4-, and 2.1-fold, respectively, while CD40L increased survival 9.2-, 9.7-, and 6.3-fold. (c) CFSE-labeled memory B cells were cultured for 5 days with media alone (squares), CD40L (diamonds), or BAFF (circles). The percentage of cells in each division was determined by division slicing. These results are representative of three different experiments.