Notch ligation by Delta1 inhibits peripheral immune responses to transplantation antigens by a CD8⁺ cell–dependent mechanism
J. Clin. Invest. Kenneth K. Wong, et al. 112:1741 doi:10.1172/JCI18020 [Go to this article.]

Figure 6
CD8⁺ cell depletion reverses Dl1 enhancement of graft survival, and purified CD8⁺ splenic cells make altered cytokine responses upon ligation with Dl1. (a) Animals were pretreated with K^b + A^b or K^b/Dl1 + A^b/Dl1 as in Figure 5. All cell injections were given intraperitoneally. One day before and on the day of transplantation animals received 1 mg CD8-depleting (YTS169) or CD4-depleting (YTS 191) Ab intraperitoneally. Graft survival was monitored by palpation, n = 4–6/group. (b) Purified splenic CD8⁺ cells were cocultured with immobilized anti-CD3 (0.04 μg/ml), soluble anti-CD28, and immobilized Dl1-Fc or IgG-4 control protein at the concentrations shown. Supernatants were harvested after 5 days and tested in ELISA for cytokines as shown. One representative experiment of three is shown.