Notch ligation by Delta1 inhibits peripheral immune responses to transplantation antigens by a CD8⁺ cell–dependent mechanism
J. Clin. Invest. Kenneth K. Wong, et al. 112:1741 doi:10.1172/JCI18020 [Go to this article.]

Figure 4
Kinetics of cell accumulation and apoptosis in PLN assay. C3H/HeJ mice were first injected with PBS, mixtures of K^b + A^b, or mixtures of K^b/Dl1 + A^b/Dl1 in the LFPs, as in Figure 1. After 14 days, they were challenged with irradiated allogeneic LNCs of H-2^b origin. At various time points after the second injection, PLNs were harvested for analysis of cell numbers (a) expressed as the ratio of mean cell numbers (LPLN/RPLN ± SEM) (b) expressed as ratio of mean T cell numbers (LPLN/RPLN ± SEM), and (c) annexin V staining. Data are expressed as the percentage of CD3⁺ cells coexpressing annexin V. Results of two separate experiments are pooled such that n = 3–6 animals per time point.