Notch ligation by Delta1 inhibits peripheral immune responses to transplantation antigens by a CD8⁺ cell–dependent mechanism
J. Clin. Invest. Kenneth K. Wong, et al. 112:1741 doi:10.1172/JCI18020 [Go to this article.]

Figure 3
Notch ligand transfectants are not immunogenic and can inhibit the response to a challenge with antigen in a PLN assay. (a) PBS, H-2^b LNCs, A^b, or A^b/Dl1 cells were injected into the LFP of C3H/HeJ mice. After 14 days, animals were challenged in the LFP with irradiated allogeneic LNCs (H-2^b, from C57BL/10), A^b, or A^b/Dl1 cells, and after an additional 7 days, PLNs from both sides were harvested, weighed, and the results expressed as a ratio of weight (LPLN/RPLN). (b) A mixed population of either K^b + A^b or K^b/Dl1 + A^b/Dl1 was used as the first injection, and a second challenge with H-2^b LNCs was given as above. PLNs were harvested and data expressed as indicated above. (c) Mixed populations of cells or PBS were given as a first injection as shown. Animals were challenged with H-2^b or H-2^d (third party–derived) LNCs. PLNs were harvested and data expressed as indicated above. (d) Either K^b + A^b or K^b/Dl1 + A^b/Dl1 were given as a first injection. DCs were used to test responsiveness to directly (direct, C57BL/10 DCs) or indirectly (indirect, C3H/HeJ DCs fed with C57BL/10 DCs) presented antigen. To check that the feeding protocol did not alter the function of DCs, control injections were with C57BL/10 DCs fed with C57BL/10 DCs (direct control) and C3H/HeJ DCs fed with C3H/HeJ DCs (syn control). Syn, syngeneic. n = 3–4 in all groups; experiments are representative of at least three repeats.