Notch ligation by Delta1 inhibits peripheral immune responses to transplantation antigens by a CD8⁺ cell–dependent mechanism
J. Clin. Invest. Kenneth K. Wong, et al. 112:1741 doi:10.1172/JCI18020 [Go to this article.]

Figure 1
Characterization of L cell transfectants. (a) MHC class I and II (K^b and A^b, respectively) and MHC Dl1-transfected (K^b/Dl1 and A^b/Dl1) cells were analyzed using flow cytometry. EGFP expression is shown on FL1-H and indicates the level of expression of the transfected Dl1:EGFP bicistronic construct. EGFP expression reflects Dl1 expression. FL1-H, level of green fluorescence. (b) Quantitative RT-PCR for Dl1 transcripts in parental K^b and A^b cells and their Dl1-transfected counterparts. All samples were normalized by assessment of 18s RNA. Mouse embryonic RNA (day 11) was used as a positive control in the experiment, and transcript levels in other samples are expressed relative to this. (c) Flow-cytometric analysis of MHC class I (H-2K^b, left panels) and MHC class II (H-2A^b, right panels) expression on all cell lines using biotinylated AF6-88 (MHC class I) and 1D9 (MHC class II) mAb’s (filled traces) or no first-stage Ab (open traces). All data are representative of at least three repeat experiments. FL2-H, level of red fluorescence.