Polymorphisms in Chlamydia trachomatis tryptophan synthase genes differentiate between genital and ocular isolates
J. Clin. Invest. Harlan D. Caldwell, et al. 111:1757 doi:10.1172/JCI17993 [Go to this article.]

Figure 1
(a) Alignment of partial sequences from the trpA gene from the14 human C. trachomatis reference serovars. A ClustalW alignment of the nucleotide regions containing sequence polymorphisms is illustrated. As compared with genital serovars, ocular serovars have a three-base (nucleotides 408–410) deletion that results in the loss of a phenylalanine. The various serovars have been grouped, in accordance with their nucleotide mutational “hot-spot” sequence. The ocular serovars have a single-base deletion (nucleotide 528) resulting in a nonfunctional truncated TrpA protein. Genital serovar specific missense mutations (nucleotides 530 and 532) that result in amino acid changes in loop 6 of TrpA are shown below the nucleotide sequence. See Fehlner-Gardiner et al. (16) for details. (b) Schematic summary of the tryptophan synthase inactivating mutations identified in clinical ocular serovars and the missense mutations identified in the clinical genital serovars.