Mice deficient in α-actinin-4 have severe glomerular disease
J. Clin. Invest. Claudine H. Kos, et al. 111:1683 doi:10.1172/JCI17988 [Go to this article.]

Figure 1
(a) α-Actinin-4 expression in mouse kidney shown at low power (image obtained at ×10 magnification). (b) Analysis of α-actinin-1, -2, -3, and -4 expression (red) and synaptopodin (synpo; green) in mouse kidney. Merged images are shown below. α-Actinin-1 and -4 show essentially podocyte-limited expression, whereas α-actinin-2 shows a different expression pattern, consistent with a mesangial cell localization. α-Actinin-3 is not expressed in the mouse glomerulus. α-Actinin-1, -2, and -4 are also expressed in the renal vasculature (not shown). Images were taken at ×40 magnification. (c) Northern blot showing expression of α-actinin-4 transcript in various mouse organs (using mouse multiple-tissue Northern blot from CLONTECH Laboratories Inc.). Lane 1, heart; 2, brain; 3, spleen; 4, lung; 5, liver; 6, skeletal muscle; 7, kidney; 8, testis. Hybridization to a β-actin control probe is shown below, with a single 2-kb band in most lanes and an expected 1.8-kb band present in heart, skeletal muscle, and testis. (d) Northern blot showing expression of α-actinin-4 at embryonic days 7, 11, 15, and 17. Hybridization to a β-actin control probe is shown below.