PTG gene deletion causes impaired glycogen synthesis and developmental insulin resistance
J. Clin. Invest. Sean M. Crosson, et al. 111:1423 doi:10.1172/JCI17975 [
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Figure 4Liver insulin signaling is not impaired in
PTG+/– mice. Protein lysates were prepared from liver of 3- to 4-month-old fasting male animals either in the basal state after intravenous injection with PBS or after intravenous injection with 2 mU/g body weight human recombinant insulin. Western blotting was performed for total and phosphospecific Akt. Liver homogenates were immunoprecipitated with an antibody against IRS-1 or IRS-2 and immunoblotting was performed to determine the levels of total IRS-1 and IRS-2 brought down in the immunoprecipitation and for levels of tyrosine-phosphorylated IRS-1 or IRS-2.
