Dissection of experimental asthma with DNA microarray analysis identifies arginase in asthma pathogenesis
J. Clin. Invest. Nives Zimmermann, et al. 111:1863 doi:10.1172/JCI17912 [Go to this article.]

Figure 4
Arginase I mRNA in situ hybridization. The hybridization signal of the arginase I antisense (AS) and sense (S) probes are shown for OVA/alum sensitized mice challenged with two doses of OVA (a–c, e) or saline (d). Tissue was analyzed 18 hours after the second saline or allergen challenge. Bright field (b, e, f) and dark field images (a, c, d) are shown at 100 × (a–d) and 400 × (e, f) original magnification. The dark field signal is white/pink and the bright field signal is black. In the paired dark and bright field photomicrographs (a and b), a peribronchial staining pattern is shown. The hybridization of the AS probe to a subpopulation of isolated inflammatory cells is shown in e. Staining was also observed in isolated large mononuclear cells with abundant cytoplasm, typical for airway macrophages. Examples of such cells stained by in situ (f) and immunohistochemistry against arginase I (g) are shown. Arrows indicate representative positive signal. Representative photomicrographs of four separate mice are shown.