Dissection of experimental asthma with DNA microarray analysis identifies arginase in asthma pathogenesis
J. Clin. Invest. Nives Zimmermann, et al. 111:1863 doi:10.1172/JCI17912 [
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Figure 1Microarray analysis of experimental asthma. In
a, the induction of eotaxin-1 in allergen-challenged mice as measured by Northern blot analysis is shown. Total RNA (10 μg) was electrophoresed, transferred, and hybridized with a radiolabeled eotaxin-1 cDNA probe. The location of 18S RNA is shown. Each lane represents a separate mouse. Ethidium bromide (EtBr) staining of the RNA gel is also shown. In
b, scatter plots of the average difference of present genes in two representative saline-challenged (left) or OVA-challenged (right) samples are shown. In
c, the average difference of present genes in a representative saline-treated sample compared with a representative OVA-treated sample is shown. In
d, quantitative analysis of the eotaxin-1 signal for saline- and OVA-treated mice is shown (
n = 3 mice each). Error bars represent the SD. In
e, the number of genes increased in experimental asthma induced with OVA or
A. fumigatus antigen is depicted in a Venn diagram. Data are derived from statistically significant bioinformatic analysis as described in the Methods section. Data are available in supplementary Tables 1–4 (http://www.jci.org/cgi/content/full/111/12/1863/DC1).
