Human phospholamban null results in lethal dilated cardiomyopathy revealing a critical difference between mouse and human
J. Clin. Invest. Kobra Haghighi, et al. 111:869 doi:10.1172/JCI17892 [Go to this article.]

Figure 4
Effect of PLN-L39stop mutant on adult rat cardiac myocyte mechanics and Ca²⁺ kinetics. Shown are representative recordings of cell shortening (a) and the Ca²⁺ transient (b) in cardiomyocytes isolated from adult rat hearts infected with Ad.GFP, Ad.PLN-WT, and Ad.PLN-L39stop. Myocytes were stimulated at 0.5 Hz at 25°C. (c) Percent myocyte fractional shortening (FS%), rate of contraction (+dL/dt), and rate of relaxation (–dL/dt). (d) Ca²⁺ transient amplitude (340/380 nm ratio) and time to 50% decay of the Ca²⁺ signal (T₅₀). A minimum of 16–20 cells was studied from each of three individual preparations. Values are means ± SEM; *P < 0.05.